bauhinia purpurea lectin (Vector Laboratories)

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Vector Laboratories bauhinia purpurea lectin
Bauhinia Purpurea Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/L-1280-5/Unconjugated+Bauhinia+Purpurea+Lectin+(BPL)/pmc04324811-41-15-19
Average 92 stars, based on 16 article reviews

bauhinia purpurea lectin - by Bioz Stars, 2026-10

92/100 stars

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Article Title: Investigation of structural and saccharide binding transitions of Bauhinia purpurea and Wisteria floribunda lectins.
Article Snippet: Two novel medicinally important legume lectins from Bauhinia purpurea (BPL) and Wisteria floribunda (WFL) possessing extended sugar binding site were investigated for functional and conformational transitions using biochemical and biophysical techniques as well as bioinformatical tools.. Homology model of BPL was constructed using the Schrodinger suite and docked with N-acetyl galactosamine and T-antigen disaccharide (Galβ13GalNAcαO-Me).. The longer loop D in the structure of WFL compared to that in BPL was found to be responsible for its specificity to LacdiNac (β-D-GalNAc-[1→ 4]-DGlcNAc) over Galβ1-3GalNAc.

Article Title: Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray
Article Snippet: High-performance liquid chromatography (HPLC) solvents were purchased from Thermo Fisher Scientific (Waltham, MA).

Article Title: Enrichment and identification of glycoproteins in human saliva using lectin magnetic bead arrays.
Article Snippet: LectinsdAleuria aurantia lectin (AAL), Bauhinia purpurea lectin (BPL), Jacalin (JAC), soybean agglutinin (SBA), Narcissus pseudonarcissus lectin (NPL), and Sambucus nigra agglutinin (SNA)dwere purchased from Vector Laboratories (Burlingame, CA, USA), whereas lectins concanavalin A (ConA) and Erythrina crista-galli agglutinin (ECA) were purchased from Sigma (St. Louis, MO, USA).

Binding Assay:

Article Title: Production of extracellular matrices during development of infection structures by the downy mildew Peronospora parasitica.
Article Snippet: .. For immunogold localization of Bauhinia purpurea agglutinin (BPA) binding sites, sections mounted on uncoated 400 mesh nickel grids were immersed in drops of the following reagents: PBS containing 1% BSA and 0.2% (v/v) Tween 20 (PBSA, 15 min); unconjugated BPA (Vector Laboratories) diluted to 50 μg ml–1 in PBSA (1 h); PBSA (5 × 3 min); rabbit anti-BPA antibodies (Vector Laboratories) diluted 1 : 100 in PBSA (1 h); PBSA (5 × 3 min); goat antirabbit IgG antibodies conjugated with 10 nm colloidal gold particles (British Biocell International) diluted 1 : 50 in PBSA (1 h); PBSA (5 × 3 min); deionized water (15 min). ..

Suspension:

Article Title: In Vitro Metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Clinical Field Isolates, as Evaluated by Morphology, Complement Resistance, and Infectivity to Human Macrophages
Article Snippet: .. L. braziliensis and L. amazonensis suspension (300 μ L) were incubated with 100 μ g/mL Bauhinia purpurea lectin (BPL, Vector Laboratory, Burlingame, CA, USA), which binds to galactosyl ( β -1,3) N-acetylgalactosamine ( β -gal (1–3)-GalNAc) present in LPG of procyclic promastigotes or 100 μ g/mL 3A1-La mAb, which binds to LPG of procyclic promastigotes, but not of metacyclic forms [ , , ], respectively. ..

Incubation:

Whole Genome Amplification:

Article Title: Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray
Article Snippet: PNGase F was purchased from New England BioLabs (Ipswich, MA), and PNGase A was from Sigma-Aldrich. .. Plant lectins were purchased from Vector Laboratories (Burlingame, CA), including Phaseolus vulgaris agglutinin L (PHA-L), Phaseolus vulgaris agglutinin E (PHA-E), peanut agglutinin (PNA), Wisteria floribunda agglutinin (WFA), wheat germ agglutinin (WGA), Lotus tetragonolobus lectin (LTL), Aleuria aurantia lectin (AAL), Sambucus nigra agglutinin (SNA), Ricinus communis agglutinin I (RCA I), concanavalin A (ConA), Ulex europaeus I (UEA I), Maackia amurensis agglutinin (MAA), Bauhinia purpurea lectin (BPL), and Griffonia simplicifolia lectin-I (GSI-B4). .. The lectin HPA ( Helix pomatia agglutinin) was purchased from Sigma-Aldrich.

Article Title: Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray
Article Snippet: PNGase F was purchased from New England Biolabs (Ipswich, MA) and PNGase A was from 118 Sigma-Aldrich. .. Plant lectins were purchased from Vector Labs (Burlingame, CA) including: PHA-L, 119 Phaseolus vulgaris agglutinin L; PHA-E, Phaseolus vulgaris agglutinin E; PNA, peanut agglutinin; 120 WFA, Wisteria floribunda agglutinin; WGA, wheat germ agglutinin; LTL, Lotus tetragonolobus lectin; 121 AAL, Aleuria aurantia lectin; SNA, Sambucus nigra agglutinin; RCA I, Ricinus communis agglutinin I; 122 ConA, concanavalin A; UEA I, Ulex europaeus I; MAA, Maackia amurensis agglutinin, BPL, Bauhinia 123 purpurea lectin, and GSI-B4, Griffonia simplicifolia lectin-I. .. The lectin HPA, Helix pomatia agglutinin 124 was purchased from Sigma-Aldrich.

Membrane:

Article Title: Quantification of CSF cystatin C using liquid chromatography tandem mass spectrometry.
Article Snippet: Background: Cystatin C (CST3), a ubiquitously expressed cysteine protease inhibitor, is implicated in several neurological diseases.. Here, we have developed an accurate CST3 measurement system based on liquid chromatography tandem mass spectrometry (LC-MS/MS).. Methods: LC-MS/MS based measurement for CSF CST3 was validated by determination of assay precision, accuracy and recovery.

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